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N plasma (A) or plasma deficient in plasminogen (B). The chromogenic substrate S-2302, specific for PK/FXIIa, was added and, after incubation, hydrolysis was determined at 405 nm. (C) Plasmin activity was detected in normal and plasminogen-deficient plasma after incubation with supernatants from overnight cultures of M49 wild-type (M49) or ska mutant ( ska) strains or purified streptokinase (pSK).

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